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Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction.

Several simplified methods for treating mycobacteria to release DNA for amplification by the polymerase chain reaction (PCR) were investigated. The most effective of the methods was sonication. Samples were placed in screw-capped microcentrifuge tubes that were then placed in a plastic rack. The rac...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Buck, G E, O'Hara, L C, Summersgill, J T
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265279/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1583142/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.5.1331-1334.1992
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