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Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction.

Several simplified methods for treating mycobacteria to release DNA for amplification by the polymerase chain reaction (PCR) were investigated. The most effective of the methods was sonication. Samples were placed in screw-capped microcentrifuge tubes that were then placed in a plastic rack. The rac...

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Dades bibliogràfiques
Publicat a:J Clin Microbiol
Autors principals: Buck, G E, O'Hara, L C, Summersgill, J T
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265279/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1583142/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.5.1331-1334.1992
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