Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function

We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a double-helix point spread function (DH-PSF). The DH-PSF design features high and uniform Fisher information and has 2 dominant lobes in the image plan...

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Main Authors: Pavani, Sri Rama Prasanna, Thompson, Michael A., Biteen, Julie S., Lord, Samuel J., Liu, Na, Twieg, Robert J., Piestun, Rafael, Moerner, W. E.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2009
Teme:
Physical Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2651341/
https://ncbi.nlm.nih.gov/pubmed/19211795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0900245106
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