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The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation

Single-molecule-based super-resolution fluorescence microscopy has recently been developed to surpass the diffraction limit by roughly an order of magnitude. These methods depend on the ability to precisely and accurately measure the position of a single-molecule emitter, typically by fitting its em...

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Detalhes bibliográficos
Main Authors: Backlund, Mikael P., Lew, Matthew D., Backer, Adam S., Sahl, Steffen J., Grover, Ginni, Agrawal, Anurag, Piestun, Rafael, Moerner, W. E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013112/
https://ncbi.nlm.nih.gov/pubmed/24817798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/12.2001671
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