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Simultaneous, accurate measurement of the 3D position and orientation of single molecules

Recently, single molecule-based superresolution fluorescence microscopy has surpassed the diffraction limit to improve resolution to the order of 20 nm or better. These methods typically use image fitting that assumes an isotropic emission pattern from the single emitters as well as control of the e...

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Bibliographic Details
Main Authors: Backlund, Mikael P., Lew, Matthew D., Backer, Adam S., Sahl, Steffen J., Grover, Ginni, Agrawal, Anurag, Piestun, Rafael, Moerner, W. E.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2012
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3511094/
https://ncbi.nlm.nih.gov/pubmed/23129640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1216687109
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