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Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a double-helix point spread function (DH-PSF). The DH-PSF design features high and uniform Fisher information and has 2 dominant lobes in the image plan...
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| Päätekijät: | , , , , , , , |
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| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
National Academy of Sciences
2009
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2651341/ https://ncbi.nlm.nih.gov/pubmed/19211795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0900245106 |
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