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Expression, purification and preliminary diffraction studies of CmlS

CmlS, a flavin-dependent halogenase (FDH) present in the chloramphenicol-biosynthetic pathway in Streptomyces venezuelae, directs the dichlorination of an acetyl group. The reaction mechanism of CmlS is of considerable interest as it will help to explain how the FDH family can halogenate a wide rang...

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Détails bibliographiques
Auteurs principaux: Latimer, Ryan, Podzelinska, Kateryna, Soares, Alexei, Bhattacharya, Anupam, Vining, Leo C., Jia, Zongchao, Zechel, David L.
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2650468/
https://ncbi.nlm.nih.gov/pubmed/19255478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108043091
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