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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes

The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal...

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Detalhes bibliográficos
Main Authors: Jackson, Colin J., Carr, Paul D., Kim, Hye-Kyung, Liu, Jian-Wei, Ollis, David L.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242963/
https://ncbi.nlm.nih.gov/pubmed/16820687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106020021
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