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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes

The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal...

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Bibliografski detalji
Glavni autori: Jackson, Colin J., Carr, Paul D., Kim, Hye-Kyung, Liu, Jian-Wei, Ollis, David L.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2006
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242963/
https://ncbi.nlm.nih.gov/pubmed/16820687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106020021
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