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Robust production and passaging of infectious HPV in squamous epithelium of primary human keratinocytes

Using Cre-loxP-mediated recombination, we established a highly efficient and reproducible system that generates autonomous HPV-18 genomes in primary human keratinocytes (PHKs), the organotypic raft cultures of which recapitulated a robust productive program. While E7 promoted S-phase re-entry in num...

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Detalhes bibliográficos
Main Authors: Wang, Hsu-Kun, Duffy, Aaron A., Broker, Thomas R., Chow, Louise T.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2648537/
https://ncbi.nlm.nih.gov/pubmed/19131434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1735109
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