Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12.

A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the lambda replacement vector L47.1. Phages expressing alpha-hemolysin were detected by overlaying plaques for...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Kehoe, M, Duncan, J, Foster, T, Fairweather, N, Dougan, G
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264614/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6350179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.41.3.1105-1111.1983
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