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Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12.

A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the lambda replacement vector L47.1. Phages expressing alpha-hemolysin were detected by overlaying plaques for...

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Publicat a:Infect Immun
Autors principals: Kehoe, M, Duncan, J, Foster, T, Fairweather, N, Dougan, G
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1983
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC264614/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6350179/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.41.3.1105-1111.1983
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