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Cloning, expression, and mapping of the Staphylococcus aureus alpha-hemolysin determinant in Escherichia coli K-12.

A fragment of Staphylococcus aureus DNA encoding the alpha-hemolysin determinant was cloned from strain Wood 46 by inserting Sau3A-generated genomic DNA fragments between the BamHI sites of the lambda replacement vector L47.1. Phages expressing alpha-hemolysin were detected by overlaying plaques for...

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Bibliografiske detaljer
Main Authors: Kehoe, M, Duncan, J, Foster, T, Fairweather, N, Dougan, G
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC264614/
https://ncbi.nlm.nih.gov/pubmed/6350179
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