A Formaldehyde-based Whole-Mount In Situ Hybridization Method for Planarians

Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types. Each model organism presents its own unique set of challenges for achieving robust and reproducible staining with cellular resolution. Here we describe a formaldehy...

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Autori principali: Pearson, Bret J., Eisenhoffer, George T., Gurley, Kyle A., Rink, Jochen C., Miller, Diane E., Alvarado, Alejandro Sánchez
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2640425/
https://ncbi.nlm.nih.gov/pubmed/19161223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.21849
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