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A Formaldehyde-based Whole-Mount In Situ Hybridization Method for Planarians

Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types. Each model organism presents its own unique set of challenges for achieving robust and reproducible staining with cellular resolution. Here we describe a formaldehy...

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Detalhes bibliográficos
Main Authors: Pearson, Bret J., Eisenhoffer, George T., Gurley, Kyle A., Rink, Jochen C., Miller, Diane E., Alvarado, Alejandro Sánchez
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2640425/
https://ncbi.nlm.nih.gov/pubmed/19161223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.21849
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