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Simultaneous Detection of FISH Signals and Bromo-Deoxyuridine Incorporation in Fixed Tissue Cultured Cells

FISH (Fluorescence in situ hybridization) is a powerful technique that detects and localises specific DNA sequences on metaphase chromosomes, interphase nuclei or chromatin fibres. When coupled to BrdU (5-Bromo 2-deoxy-uridine) labeling of newly replicated DNA, the replication properties of differen...

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Bibliografiske detaljer
Main Authors: Moralli, Daniela, Monaco, Zoia L.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2637417/
https://ncbi.nlm.nih.gov/pubmed/19221585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0004483
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