Flow Cytometry for Real-Time Measurement of Guanine Nucleotide Binding and Exchange by Ras-like GTPases

Ras-like small GTPases cycle between GTP-bound active and GDP-bound inactive conformational states to regulate diverse cellular processes. Despite their importance, detailed kinetic or comparative studies of family members are rarely undertaken due to the lack of real-time assays measuring nucleotid...

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Main Authors: Schwartz, Samantha L., Tessema, Mathewos, Buranda, Tione, Phlypenko, Olena, Rak, Alexey, Simons, Peter C., Surviladze, Zurab, Sklar, Larry A., Wandinger-Ness, Angela
Format: Artigo
Sprog:Inglês
Udgivet: 2008
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2633595/
https://ncbi.nlm.nih.gov/pubmed/18638444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.06.039
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