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An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Toward the expansion of the genetic alphabet, we present an unnatural base pair system for efficient PCR amplification, enabling the site-specific incorporation of extra functional components into DNA. This system can be applied to conventional PCR protocols employing DNA templates containing unnatu...

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Autors principals: Kimoto, Michiko, Kawai, Rie, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2632903/
https://ncbi.nlm.nih.gov/pubmed/19073696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn956
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