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Design of an Optical Switch for Studying Conformational Dynamics in Individual Molecules of GroEL
We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulatio...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2008
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2632776/ https://ncbi.nlm.nih.gov/pubmed/18572960 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc800118j |
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