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Design of an Optical Switch for Studying Conformational Dynamics in Individual Molecules of GroEL
We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulatio...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2632776/ https://ncbi.nlm.nih.gov/pubmed/18572960 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc800118j |
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