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Refinement of non-calorimetric determination of the change in heat capacity, ΔCp, of protein unfolding and validation across a wide temperature range

The change in heat capacity, ΔC(p), upon protein unfolding has been usually determined by calorimetry. A non-calorimetric method which employs the Gibbs-Helmholtz relationship to determine ΔC(p) has seen some use. Generally, the free energy change upon unfolding of the protein is determined at a var...

詳細記述

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書誌詳細
主要な著者: Singh, Deepika Talla, Stites, Wesley E.
フォーマット: Artigo
言語:Inglês
出版事項: 2008
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2630543/
https://ncbi.nlm.nih.gov/pubmed/18384147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.22016
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