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Refinement of non-calorimetric determination of the change in heat capacity, ΔCp, of protein unfolding and validation across a wide temperature range
The change in heat capacity, ΔC(p), upon protein unfolding has been usually determined by calorimetry. A non-calorimetric method which employs the Gibbs-Helmholtz relationship to determine ΔC(p) has seen some use. Generally, the free energy change upon unfolding of the protein is determined at a var...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2008
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2630543/ https://ncbi.nlm.nih.gov/pubmed/18384147 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prot.22016 |
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