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Stoichiometry of molecular complexes at adhesions in living cells

We describe a method to detect molecular complexes and measure their stoichiometry in living cells from simultaneous fluctuations of the fluorescence intensity in two image channels, each detecting a different kind of protein. The number and brightness (N&B) analysis, namely, the use of the rati...

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Autors principals: Digman, Michelle A., Wiseman, Paul W., Choi, Colin, Horwitz, Alan R., Gratton, Enrico
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2630200/
https://ncbi.nlm.nih.gov/pubmed/19168634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0806036106
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