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Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method

We describe a general method for detecting molecular complexes based on the analysis of single molecule fluorescence fluctuations from laser scanning confocal images. The method detects and quantifies complexes of two different fluorescent proteins noninvasively in living cells. Because in a raster...

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Detalles Bibliográficos
Autores principales: Digman, Michelle A., Wiseman, Paul W., Horwitz, Alan R., Gratton, Enrico
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716688/
https://ncbi.nlm.nih.gov/pubmed/19167315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.09.051
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