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Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method

We describe a general method for detecting molecular complexes based on the analysis of single molecule fluorescence fluctuations from laser scanning confocal images. The method detects and quantifies complexes of two different fluorescent proteins noninvasively in living cells. Because in a raster...

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Detalhes bibliográficos
Main Authors: Digman, Michelle A., Wiseman, Paul W., Horwitz, Alan R., Gratton, Enrico
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716688/
https://ncbi.nlm.nih.gov/pubmed/19167315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.09.051
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