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Systematic Method for Determining an Ideal Housekeeping Gene for Real-Time PCR Analysis
To standardize the amount of biological material between samples (e.g., number of cells or amount of tissue) for quantitative real-time reverse transcriptase PCR (qRT-PCR), the cycle of the target gene at which expression is detected (the cycle threshold, or Ct) is divided by the Ct of a gene either...
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| Hoofdauteurs: | , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
The Association of Biomolecular Resource Facilities
2008
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2628067/ https://ncbi.nlm.nih.gov/pubmed/19183798 |
| Tags: |
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