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Diurnal variation in opsin expression and common housekeeping genes necessitates comprehensive normalization methods for quantitative real-time PCR analyses
To determine the visual sensitivities of an organism of interest, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is often used to quantify expression of the light-sensitive opsins in the retina. While qRT-PCR is an affordable, high-throughput method for measuring expression,...
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| Publicado no: | Mol Ecol Resour |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6995727/ https://ncbi.nlm.nih.gov/pubmed/31325910 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1755-0998.13062 |
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