High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Described in this report is a qRT-PCR assay for the analysis of seventeen human IFN subtypes in a 384-well plate format that incorporates highly specific locked nucleic acid (LNA) and molecular beacon (MB) probes, transcript standards, automated multichannel pipetting, and plate drying. Determining...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Renn, Lynnsey A., Theisen, Terence C., Navarro, Maria B., Mane, Viraj P., Schramm, Lynnsie M., Kirschman, Kevin D., Fabozzi, Giulia, Hillyer, Philippa, Puig, Montserrat, Verthelyi, Daniela, Rabin, Ronald L.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401384/
https://ncbi.nlm.nih.gov/pubmed/25867042
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52650
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