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Alternate (13)C-(12)C labeling for complete mainchain resonance assignments using Cα direct-detection with applicability towards fast relaxing protein systems

[Image: see text] Experiments that use direct (13)C detection and take advantage of the slower relaxation of (13)C magnetization compared to 1H offer an attractive strategy for extending the limits of NMR to include larger, highly dynamic, or paramagnetic proteins. Because carbonyl carbons ((13)C′)...

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Hlavní autoři: Takeuchi, Koh, Sun, Zhen-Yu J., Wagner, Gerhard
Médium: Artigo
Jazyk:Inglês
Vydáno: 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2626166/
https://ncbi.nlm.nih.gov/pubmed/19049287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja806956p
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