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DNA Sequencing By Denaturation: Principle and Thermodynamic Simulations

We describe a new DNA sequencing method called sequencing by denaturation (SBD). A Sanger dideoxy sequencing reaction is performed on the templates on a solid surface to generate a ladder of DNA fragments randomly terminated by fluorescently-labeled dideoxyribonucleotides. The labeled DNA fragments...

詳細記述

保存先:
書誌詳細
主要な著者: Chen, Ying-Ja, Huang, Xiaohua
フォーマット: Artigo
言語:Inglês
出版事項: 2008
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2621085/
https://ncbi.nlm.nih.gov/pubmed/18930015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.09.048
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