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DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device

We report the proof of concept of a novel DNA sequencing technology called sequencing by denaturation (SBD). SBD is based on the Sanger sequencing reaction performed on amplified target templates immobilized on a solid surface followed by the denaturation of these Sanger fragments. As these fluoresc...

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Detalhes bibliográficos
Main Authors: Chen, Ying-Ja, Roller, Eric E., Huang, Xiaohua
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2881221/
https://ncbi.nlm.nih.gov/pubmed/20390134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b921417h
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