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DNA Sequencing By Denaturation: Principle and Thermodynamic Simulations
We describe a new DNA sequencing method called sequencing by denaturation (SBD). A Sanger dideoxy sequencing reaction is performed on the templates on a solid surface to generate a ladder of DNA fragments randomly terminated by fluorescently-labeled dideoxyribonucleotides. The labeled DNA fragments...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2621085/ https://ncbi.nlm.nih.gov/pubmed/18930015 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.09.048 |
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