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Mapping RNA exit channel on transcribing RNA polymerase II by FRET analysis

A simple genetic tag-based labeling method that permits specific attachment of a fluorescence probe near the C terminus of virtually any subunit of a protein complex is implemented. Its immediate application to yeast RNA polymerase II (pol II) enables us to test various hypotheses of RNA exit channe...

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Hlavní autoři: Chen, Chin-Yu, Chang, Chia-Chi, Yen, Chi-Fu, Chiu, Michael T.-K., Chang, Wei-Hau
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2610012/
https://ncbi.nlm.nih.gov/pubmed/19109435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0811689106
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