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Mapping RNA exit channel on transcribing RNA polymerase II by FRET analysis
A simple genetic tag-based labeling method that permits specific attachment of a fluorescence probe near the C terminus of virtually any subunit of a protein complex is implemented. Its immediate application to yeast RNA polymerase II (pol II) enables us to test various hypotheses of RNA exit channe...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2610012/ https://ncbi.nlm.nih.gov/pubmed/19109435 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0811689106 |
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