Transposon Tn5 mutagenesis of Brucella abortus.

We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 2...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Smith, L D, Heffron, F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC259975/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822580/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.11.2774-2776.1987
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