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Transposon Tn5 mutagenesis of Brucella abortus.

We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 2...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Infect Immun
Prif Awduron: Smith, L D, Heffron, F
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology (ASM) 1987
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC259975/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822580/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.55.11.2774-2776.1987
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