Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA contain...

Full description

Saved in:
Bibliographic Details
Main Authors: Yamamoto, M, Jones, J M, Senghas, E, Gawron-Burke, C, Clewell, D B
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC203811/
https://ncbi.nlm.nih.gov/pubmed/3038014
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items