Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA contain...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Yamamoto, M, Jones, J M, Senghas, E, Gawron-Burke, C, Clewell, D B
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1987
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3038014/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.5.1069-1072.1987
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