Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA contain...

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Bibliografiska uppgifter
Huvudupphovsmän: Yamamoto, M, Jones, J M, Senghas, E, Gawron-Burke, C, Clewell, D B
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1987
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC203811/
https://ncbi.nlm.nih.gov/pubmed/3038014
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