Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.
A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA contain...
Spremljeno u:
| Izdano u: | Appl Environ Microbiol |
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| Glavni autori: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1987
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3038014/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.5.1069-1072.1987 |
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