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Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification

Biologically important human proteins often require mammalian cell expression for structural studies, presenting technical and economical problems in the production/purification processes. We introduce a novel affinity peptide tagging system that uses a low affinity anti-peptide monoclonal antibody....

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Detalhes bibliográficos
Main Authors: Nogi, Terukazu, Sangawa, Takeshi, Tabata, Sanae, Nagae, Masamichi, Tamura-Kawakami, Keiko, Beppu, Ayako, Hattori, Mitsuharu, Yasui, Norihisa, Takagi, Junichi
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2590912/
https://ncbi.nlm.nih.gov/pubmed/18787202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.038299.108
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