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Tags for Labeling Protein N-termini with Subtiligase for Proteomics

The peptide ligase subtiligase, derived from subtilisin, has been employed in the identification of protein N-termini in complex mixtures. Here, the peptide ester substrates for the ligation reaction were optimized with respect to solubility, resulting in greater incorporation of the N-terminal tags...

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Detalhes bibliográficos
Main Authors: Yoshihara, Hikari A. I., Mahrus, Sami, Wells, James A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2590642/
https://ncbi.nlm.nih.gov/pubmed/18762420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2008.08.044
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