Subtiligase: a tool for semisynthesis of proteins.
A variant of subtilisin BPN', which we call subtiligase, has been used to ligate esterified peptides site-specifically onto the N termini of proteins or peptides in aqueous solution and in high yield. We have produced biotinylated or heavy-atom derivatives of methionyl-extended human growth hormone...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45475/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7809074/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.26.12544 |
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