Unexpected frameshifts from gene to expressed protein in a phage-displayed peptide library

A library of long peptides displayed on the pIII protein of filamentous phage was used in biopanning experiments against several protein targets. We find that a large percentage of phage clones that bind specifically to a target contain peptide-encoding genes that do not have an ORF. Instead, the re...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Cárcamo, Juan, Ravera, Mark W., Brissette, Renée, Dedova, Olga, Beasley, James R., Alam-Moghé, Ameena, Wan, Changhong, Blume, Arthur, Mandecki, Wlodek
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1998
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9736704/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.19.11146
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!