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Selection for improved subtiligases by phage display

Engineering enzyme activity has been challenging because of uncertainties in structure–function relationships and difficulties in screening a large number of mutant enzymes. A product capture strategy using phage display is presented here for the selection of improved enzymes from a large library of...

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Detalhes bibliográficos
Main Authors: Atwell, Shane, Wells, James A.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22237/
https://ncbi.nlm.nih.gov/pubmed/10449721
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