High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi

INTRODUCTION: Rosa26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice. However, the vectors generally used to generate Rosa26 knock-in constructs show instability problems, which have a severe impact on the efficiency...

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Bibliografski detalji
Glavni autori: Hohenstein, Peter, Slight, Joan, Ozdemir, Derya Deniz, Burn, Sally F, Berry, Rachel, Hastie, Nicholas D
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2008
Teme:
Methodology
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583990/
https://ncbi.nlm.nih.gov/pubmed/19014667
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1755-8417-1-3
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