A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular natur...

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Autors principals: Dolt, Karamjit Singh, Lawrence, Melanie L., Miller-Hodges, Eve, Slight, Joan, Thornburn, Anna, Devenney, Paul S., Hohenstein, Peter
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3639282/
https://ncbi.nlm.nih.gov/pubmed/23637962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0062054
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