Optimization of the Oligonucleotide Ligation Assay, a Rapid and Inexpensive Test for Detection of HIV-1 Drug Resistance Mutations, for Non-North American Variants

OBJECTIVE: We evaluated the feasibility of the oligonucleotide ligation assay (OLA), a specific, sensitive, and economical ligase-based point mutation assay designed to detect HIV-1 drug–resistance mutations at 12 codons of HIV-1 subtype B pol, for potential use in resource-poor settings. METHODS: S...

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Autors principals: Beck, Ingrid A., Crowell, Claudia, Kittoe, Robin, Bredell, Helba, Machaba, Molefe, Willamson, Carolyn, Janssens, Wouter, Jallow, Sabelle, van der Groen, Guido, Shao, Yiming, Jacob, Mini, Samuel, N. M., de Rivera, Ivette Lorenzana, Ngo-Giang-Huong, Nicole, Cassol, Sharon, Alemnji, George, Frenkel, Lisa M.
Format: Artigo
Idioma:Inglês
Publicat: 2008
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583356/
https://ncbi.nlm.nih.gov/pubmed/18614915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/QAI.0b013e31817ed7d7
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