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Targeted ablation of beta cells in the embryonic zebrafish pancreas using E.coli nitroreductase

In order to generate a zebrafish model of β cell regeneration, we have expressed an E.coli gene called nfsB in the β cells of embryonic zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme, which can convert prodrugs such as metronidazole (Met) to cytotoxins. By fusing nfsB to mCherr...

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Autors principals: Pisharath, Harshan, Rhee, Jerry M., Swanson, Michelle A., Leach, Steven D., Parsons, Michael J.
Format: Artigo
Idioma:Inglês
Publicat: 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583263/
https://ncbi.nlm.nih.gov/pubmed/17223324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mod.2006.11.005
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