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Thermal denaturation of wild type and mutant recombinant acetylcholinesterase from amphioxus: effects of the temperature of in vitro expression and of reversible inhibitors
We have studied the thermal inactivation at 37°C of wild type and mutant ChE2 (C310A, F312I, C466A, C310A/F312I, and C310A/C466A) from amphioxus (Branchiostoma floridae) expressed in vitro in COS-7 monkey cells under three sets of conditions: 30°C for 48 h, 30°for 24 h and C37°C for 24 h, and 37°C f...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2574716/ https://ncbi.nlm.nih.gov/pubmed/18677525 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10158-008-0075-5 |
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