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Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses

High-level production of membrane proteins, particularly of G protein-coupled receptors (GPCRs) in heterologous cell systems encounters a number of difficulties from their inherent hydrophobicity in their transmembrane domains, which frequently cause protein aggregation and cytotoxicity and thus red...

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Detalhes bibliográficos
Main Authors: Kaiser, Liselotte, Graveland-Bikker, Johanna, Steuerwald, Dirk, Vanberghem, Mélanie, Herlihy, Kara, Zhang, Shuguang
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2572932/
https://ncbi.nlm.nih.gov/pubmed/18840687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804766105
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