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A Crystallographic Study of Bright Far-Red Fluorescent Protein mKate Reveals pH-induced cis-trans Isomerization of the Chromophore

The far-red fluorescent protein mKate (λ(ex), 588 nm; λ(em), 635 nm; chromophore-forming triad Met(63)-Tyr(64)-Gly(65)), originating from wild-type red fluorescent progenitor eqFP578 (sea anemone Entacmaea quadricolor), is monomeric and characterized by the pronounced pH dependence of fluorescence,...

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Detalhes bibliográficos
Main Authors: Pletnev, Sergei, Shcherbo, Dmitry, Chudakov, Dmitry M., Pletneva, Nadezhda, Merzlyak, Ekaterina M., Wlodawer, Alexander, Dauter, Zbigniew, Pletnev, Vladimir
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2570900/
https://ncbi.nlm.nih.gov/pubmed/18682399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M800599200
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