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Understanding blue-to-red conversion in monomeric fluorescent timers and hydrolytic degradation of their chromophores

Fast-FT is a fluorescent timer (FT) engineered from DsRed-like fluorescent protein mCherry. Crystal structures of Fast-FT (chromophore Met66-Tyr67-Gly68) and its precursor with blocked blue-to-red conversion Blue102 (chromophore Leu66-Tyr67-Gly68) have been determined at the resolution of 1.15 Å and...

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Detalhes bibliográficos
Main Authors: Pletnev, Sergei, Subach, Fedor V., Dauter, Zbigniew, Wlodawer, Alexander, Verkhusha, Vladislav V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2887295/
https://ncbi.nlm.nih.gov/pubmed/20121102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja908418r
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